CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)

Cat no: C2548-83B

Supplier: United States Biological

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Additional Information

Applications: Suitable for use in flow cytometry, Immunohistochemistry None Detected Immunofluorescence Other applications have not been tested. Recommended Dilutions: Flow cytometry: after in vitro activation of T cells with selected stimuli (Concanavalin A, or solid-phase fixed-CD3 + CD28 Mabs stimulation). Immunohistochemistry: 2ug/5x10e5 cells/test. (frozen tissue sections)5-50ug/ml Immunofluorescence:2ug/5x10e5 cells/test. Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of P3/X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degrees C. The addition of 0.1% (w/v) sodium azide is recommended forReconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	C2548-83B
Hosts:	Mouse
Applications:	Flow Cytometry, Immunofluorescence, Immunohistochemistry
Size:	100ug
Form:	Supplied as a Lyophilized powder from PBS, pH 7.2 containing 1mg/ml BSA. No preservatives added. Reconstitute with 1ml ddH2O or 40-50% glycerol.
P type:	Mab
Isotype:	IgG2a
Purity:	Purified
References:	1. Brunet, J.F., et al., (1987),
Additional info:	Recognizes CD152 (CTAL-4). The

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CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)

Cat no: C2548-83B

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