CD152 IgG2a Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (Biotin)

Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses. The CD152 Ig fusion protein has biological activity and binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2). CD152 Ig will block the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) monoclonal antibodies.\n\nHuman CD152(CTLA-4)-muIg/Biotin Fusion Protein (also binds to mouse CD80/CD86)\n\nMolecular Structure: A soluble 110 kd dimeric fusion protein consisting of the extracellular (125aa) domain of human CD152 (CTLA-4) fused to murine IgG2a Fc (233aa).\n\nTransfectant Cell Line: BHK\n\nApplications: \nSuitable for use in Antibody Blocking, ELISA and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution\nFlow Cytometry: 0.5ug/ml. 80ul labels 4x10e5 Raji human tumor cells.\nAntibody Blocking: CD152 Ig binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2) (4, 5). CD152 Ig blocks the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) mAbs.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2548-83V1

Size

25ug

Applications

ELISA, FC

Conjugates

Biotin

Form

Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.

Purity

Purified by Protein A affinity chromatography.

References

1. T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. 2. T.L. Walunas, et al, (1994) Immunity 1: 405-413. 3. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. 4. A.H. Cross, et al, (1995) J Clin Invest 95: 2783-2789. 5. P.A. Morton, et al, (1996) J Immunol 156: 1047-1054.