CD152 IgG2a Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (Preservative Free)

mmune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses\n\nMolecular Structure: A soluble 110 kd dimeric fusion protein consisting of the extracellular (125aa) domain of human CTLA-4 fused to murine IgG2a Fc (233aa), with a predicted non glycosylated monomeric molecular weight of 40.1kD.\n\nTransfectant Cell Line: \nBHK\n\nApplications: \nSuitable for use in ELISA and Flow Cytometry. Other applications not tested.\n\nFunctional Application:\nBinds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2) (4, 5). Blocks the binding of anti-human CD80 (B7-1) and anti-human CD86 (B7-2) mAbs to recepters on Raji cells. Crossreacts with CD80 and CD86 across a broad range of mammalian species including pig (6).\n\nRecommended Dilution:\nFlow Cytometry: 05ug/ml. 80ul of diluted C2548-83VX labels 5x10e5 cultured Raji cells. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2548-83VX

Size

25ug

Applications

ELISA, FC

Form

Supplied as a liquid in PBS, pH 7.2, sterile filtered under aseptic conditions. No preservative added.

Purity

Purified by Protein A affinity chromatography. (> 95% by SDS-PAGE with <1% bovine immunoglobulin)

References

1. T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. \n2. T.L. Walunas, et al, (1994) Immunity 1: 405-413.\n3. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. \n4. A.H. Cross, et al, (1995) J Clin Invest 95 : 2783-2789. \n5. P.A. Morton,et al, (1996) J Immunol 156: 1047-1054. \n6. Martin K. Oaks and Karen M. Hallett, (2000) J Immunol 164: 5015-5018