CD154 cell surface antigen is a 32kD glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells. C2548-84YG also recognizes the soluble form of CD154.
Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:100; 10ul labels 10e6 cells in 100ul
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.