CD154 is a member of the TNF family of molecules. It binds to CD40 and acts as a co-stimulatory molecule by activating antigen presenting cells, such as B cells. While CD154 is mostly found expressed on activated T cells.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the myeloma Sp2/0 cell line.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.