CD157, Recombinant, Mouse

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA-derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).\n\nSource: Recombinant mouse CD157 encoded by the BST-1 gene (rmCD157; amino acid residues 25 to 285; Accession # Q64277) was expressed with a N-terminal signal peptide and a C-terminal 6X His tag in a murine myeloma cell line, NS0.\n\nDefinition: Measured by its ability to cyclize nicotinamide guanine dinucleotide (NGD+). \n\nSpecific Activity: (same/more than)10 pmol/min/ug.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Predicts 30kD and migrates as 37-, 40-, and 44kD bands in SDS-PAGE under reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2548-86J

Size

50ug

Applications

WB

Reactivities

Mouse

Form

Supplied as a liquid in 25mM Tris, 0.15M sodium chloride, pH 7.5.

Purity

(same/more than) 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin (same/less than) 1EU.

References

1. Hussain, A. M. M. et al., 1998, Protein Express. Purif. 12:133 - 137.\n2. Sato, A. et al., 1999, Biochem. J. 337:491 - 496.\n3. Kaisho, T. et al., 1994, Proc. Natl. Acad. Sci. USA 91:5325 - 5329.\n4. Ortolan, E. et al., 2002, Cell Biochem. and Function. 20:309 - 322.