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CD16

CD16

Cat no: C2267-29L

Supplier: United States Biological
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Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphtidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were incubated 45 min on ice with 80ul of anti-CD16 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 13% sub population of the cells stained positive with a mean shift of 1.54 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2267-29L
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79:3275-3279, 2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597, 3. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12:218-234, 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814, 4. C. Edberg & R.P. Kimberly, (1997) J Immunol 159:3849-3857.
Additional info: Recognizes human CD16 both allelic forms and blocks binding of complexed IgG.

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