CD16 is a low affinity Fc receptor of complexed IgG, Fc/gamma RIII and expressed on NK cells, granulocytes, activated macrophages and a subset of T cells. CD16 exists both as a glycosyl-phosphatidylinositol-anchored protein in polymorphonuclear cells and as a transmembrane protein in NK cells. This antigen will help detect CD16 and NK cells in a variety of tissues.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:20-1:40 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.
Positive Control:
Tonsil, membrane staining pattern of macrophage, neutrophils, NK cells and granulocytes.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.