Home  >  Products  >  CD16 (CD16a Antigen, CD16a, Fc-gamma RIII, FCgRIII, FcRIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcRIIIa, FCG3, FCGR3, FCGR3A, FcR-10, IGFR3, IgG Fc Receptor III-2, Low Affinity Immunoglobulin gamma Fc Region Receptor III-A)

CD16 (CD16a Antigen, CD16a, Fc-gamma RIII, FCgRIII, FcRIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcRIIIa, FCG3, FCGR3, FCGR3A, FcR-10, IGFR3, IgG Fc Receptor III-2, Low Affinity Immunoglobulin gamma Fc Region Receptor III-A)

Cat no: C2267-32N


Supplier: United States Biological
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CD16 is a low affinity Fc receptor of complexed IgG, Fc/gamma RIII and expressed on NK cells, granulocytes, activated macrophages and a subset of T cells. CD16 exists both as a glycosyl-phosphatidylinositol-anchored protein in polymorphonuclear cells and as a transmembrane protein in NK cells. This antigen will help detect CD16 and NK cells in a variety of tissues. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20-1:40 for 1 hr. at RT Optimal dilutions to be determined by the researcher. Positive Control: Tonsil, membrane staining pattern of macrophage, neutrophils, NK cells and granulocytes. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2267-32N
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry
Size: 1ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgG2a
Purity: Supernatant
Alternative names: Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a)
References: 1.Venneker G T, Das P K, Meinardi M M H M, et al.. Glycosylphosphatidylinositol (GPI)-anchored membrane proteins are constitutively down-regulated in psoriatic skin. Journal of Pathology. 172:189-197 (1994). 2.Tuijnman W B, Van Wichen D F and Schuurman H-J. Tissue distribution of human IgG Fc receptors CD16, CD32 and CD64: An immunohistochemical study. APMIS. 101:319-329 (1993).
Additional info: Recognizes CD16. Species Crossreactivity: Human.

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