CD160, Recombinant, Mouse, 6-His-Tag

CD160 (also Natural killer cell receptor BY55) is a 16kD (predicted) member of the Ig superfamily (1-4). In mouse, it is expressed principally on nonmyeloid hematopoietic cells. These include CD3+ NK1.1 cells, CD8+ TEM and TCM T cells, CD8a+ IELs, NKT cells, CD8-yd TCR T cells, and vascular endothelial cells (1, 5-7). Mouse CD160 has been identified as a 20-21kD GPI-linked glycoprotein (4, 5). It is synthesized as a preproprotein that is 185aa in length. The precursor contains a 27 aa signal sequence, a 133 aa mature molecule that shows one 98 aa V-type Ig-like domain (aa 28-125), and a 25 aa prosegment that is cleaved to generate a GPI-linkage at Ser160. Mouse GPI-linked CD160 is known to be cleaved by phospholipase C, and this generates a 40kD (presumably dimeric) band in SDS-PAGE (5). One alternative splice form for mouse CD160 is reported that appears to show a deletion of aa 137 - 180. This may generate a soluble molecule (5; GenBank Accession # NP_001156969). Mature mouse CD160 shares 63% and 88% aa identity with human and rat CD160, respectively. In mouse, CD160 is reported to bind to bind to HVEM/TNFRSF14, and both classical and non-classical MHC Class I molecules (5, 8). MHC-I proteins recognized by CD160 include Dd, Kb, Qa-1b and CD1d (5). Upon engagement, the effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to human HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on NK1.1 cells, mouse CD160 ligation alone has no effect; when combined with NK1.1 antigen stimulation, CD160 decreases NK cell IFN-y secretion. Relative to cytotoxicity, NK cell activity is positively correlated with the presence of CD160 (5).\n\nSource:\nRecombinant corresponding to 28aa from mouse CD160, fused with 6-His tag at C-Terminal, expressed in CHO cells.\n\nMolecular Weight: \n~15.8kD\n\nEntodoxin Level:\n(same/less than)1EU/1ug (LAL)\n\nBiological Activity:\nMeasured by its binding ability in a functional ELISA. When recombinant mouse HVEM Fc Chimera is immobilized at 0.5ug/ml (100ul/well), the concentration of recombinant mouse CD160 that produces 50% of the optimal binding response is found to be ~0.012-0.06ug/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2548-88P

Size

50ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute with PBS 250ug/ml.

Purity

~95% (SDS-PAGE)

References

1.Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244. 2.del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223. 3. Maiza, H. et al. (1993) J. Exp. Med. 178:1121. 4.Anumanthan, A. et al. (1998) J. Immunol. 161:2780. 5. Maeda, M. et al. (2005) J. Immunol. 175:4426. 6.Fons, P. et al. (2006) Blood 108:2608. 7. Tsujimura, K. et al. (2006) Immunol. Lett. 48:106. 8. Cheung, T.C. et al. (2009) Proc. Natl. Acad. Sci USA 106:6244.