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CD166 (ALCAM, Activated Leukocyte Cell Adhesion Molecule) (Biotin)

CD166 (ALCAM, Activated Leukocyte Cell Adhesion Molecule) (Biotin)

Cat no: C2548-90M8

Supplier: United States Biological
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Human CD166 is a type 1 glycoprotein expressed on activated T cells, B cells and monocytes and appears to be the ligand for CD6 (1,2). Human CD166 may be important for activation of T cells. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit for rhALCAM is approximately 1ng/lane under non-reducing and reducing conditions. ELISA: This antibody can be used as a detection antibody in a human ALCAM ELISA in combination with the monoclonal capture antibody (Catalog# C2548-90P). Using plates coated with 100ul/well of the capture antibody, in combination with 100ul/well of the detection antibody at 50ng/ml, an ELISA for sample volumes of 100ul can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 4ng/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2548-90M8
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human ALCAM. Less than 0.2% cross-reactivity with rmALCAM, rhICAM-2, rhICAM-3, rhE-Selectin and rhP-Selectin is observed.

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