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CD166 (ALCAM, Activated Leukocyte Cell Adhesion Molecule) (Biotin)

CD166 (ALCAM, Activated Leukocyte Cell Adhesion Molecule) (Biotin)

Cat no: C2548-90N

Supplier: United States Biological
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Human CD166 is a type 1 glycoprotein expressed on activated T cells, B cells and monocytes and appears to be the ligand for CD6 (1,2). Human CD166 may be important for activation of T cells. Applications: Suitable for use in Western Blot, Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25ug/ml and add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2548-90N
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Conjugates: Biotin
Size: 250ug
Form: Supplied as a sterile-filtered, lyophilized powder in PBS, pH 7.2, BSA. Reconstitution: sterile PBS. If 1ml of PBS is used, the antibody concentration will be 250ug/ml.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G immunoaffinity chromatography.
Additional info: Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, rmMadCAM or rmOCAM.

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