Antibodies to human CD17 react with lactosylceramide which is expressed on granulocytes, monocytes, platelets, and basophils (1). CD17 expression is downmodulated on activated granulocytes.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 5x10e5 cultured Nalm-6 human tumor cells were washed and preincubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD17 antibody at 20ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC; this reagent cross reacts well with Mouse IgM, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.53 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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