The ITGB2 protein product is the integrin beta chain beta 2. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Functional Assays and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 2ug/ml
Western Blot: 2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunoprecipitation: 2ug/ml
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.