CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2) (Biotin)

LFA-1 is a general leukocyte cell adhesion molecule, its activity being regulated by cell activation.\n\nHybridoma: \nFormed from the fusion of PAI mouse myeloma cells and LN cells from a BALB/c mouse\n\nApplication(s): \nSuitable for use in Flow Cytometry, Immunohistochemistry and Adhesion Blocking.\n\nWorking Dilution: \nFlow Cytometry: 1:50-1:100 (0.5-1ug will label about 10e6 cells)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2269-05

Size

50ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Rat

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.05% sodium azide.

P Type

Mab

Purity

(same/more than) 99% Purified by affinity chromatography. No contaminants detected. Single band by SDS-PAGE, IEP, and/or RID. The purified monoclonal antibody was biotinylated with N-hydroxysuccinimidobiotin and then dialyzed to remove remaining free biotin.

Isotype

IgG1

References

1. Tamatani, T. et al., Eur. J. Immunol., 21:627 - 633 (1991)\n2. Tamatani, T. et al., Eur. J. Immunol., 21:855 - 858 (1991)

Additional Info

Recognizes the rat LFA-1 (lymphocyte function associated molecule-1) beta chain (CD18; 95-100kD) which is associated with the alpha chain (CD11a; 160-70kD). Therefore, this monoclonal antibody recognizes Mac-1 and gp150/95 as well as LFA-1. Inhibits homotypic aggregation of PHA blasts.It also blocks binding of rat lymphocytes to purified rat ICAM-1.