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CD19 (B4, B-Lymphocyte Antigen CD19, B-Lymphocyte Surface Antigen B4, CVID3, Differentiation Antigen CD19, MGC12802, T-Cell Surface Antigen Leu-12)

CD19 (B4, B-Lymphocyte Antigen CD19, B-Lymphocyte Surface Antigen B4, CVID3, Differentiation Antigen CD19, MGC12802, T-Cell Surface Antigen Leu-12)

Cat no: C2269-50V

Supplier: United States Biological
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Human CD19 is a B cell restricted membrane protein expressed from early stages of B cell development and associates with the antigen receptor complex. CD19 is involved in the regulation of B cell proliferation but eventually disappears from plasma cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Raji cells were washed and incubated 45 min on ice with 80ul of anti-CD19 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.7 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2269-50V
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. D.T. Fearon & R.H. Carter, (1995) Annu Rev Immunol 13:127-149, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 507-511, 3. S. Behr & F. Schriever, (1995) J Exp Med 182:1191-1199.
Additional info: Recognizes the human CD19 molecule of about 95kD and induces adhesion of B cells.

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