CD19, a 95kD cell surface glycoprotein, which is expressed by cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required
Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Western Blot: Detetcts a band at~95kDa in Raji cell lysates under reducing conditions
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab