CD19 cell surface antigen, a 96kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. Labels all B lymphocytes in peripheral blood and spleen but is negative on granulocytes, monocytes, platelets, T lymphocytes and erythrocytes. This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry and immunofluorescence.
Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:50
ELISA: 1:1000
Western Blot: 1:50-1:100
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.