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CD1w3

CD1w3

Cat no: C2251-95

Supplier: United States Biological
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Bovine CD1w3 is expressed by immature cortical thymocytes but not by mature CD2+ or WC1+ cells in peripheral lymphoid tissues. It is also expressed by B cells, monocytes and subsets of dendritic cells. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2251-95
Reactivities: Bovine, Goat, Sheep
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation
Size: 2ml
Form: Supplied as liquid, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Supernatant
References: 1. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle. Vet. Immunol. Immunopathol. 39: 25-48.2. Naessens, J. et al. (1993) Cross reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet. Immunol. Immunopathol. 39: 283-290.3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol. Today 8: 365-3
Additional info: Recognizes the bovine CD1w3 cell surface antigen, a 44kD molecule expressed as a heterodimer with beta 2 microglobulin. Species Crossreactivity: goat, sheep.

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