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CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11)

Cat no: C2253-02B


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:50 Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2253-02B
Reactivities: Bovine, Goat
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 250ul
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Ascites.
Alternative names: CD2 Molecule
References: 1. Davis, W.C. and Splitter, G.S. (1991) Individual antigens of cattle. Bovine CD2 (BoCD2). Vet. Immunol. Immunopathol. 27: 43-50, 2. Sopp, P et al. (1991) Investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence. Vet. Immunol. Immunopathol. 27: 163-168, 3. Eskra, L et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-229, 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334.
Additional info: Recognizes bovine CD2. Species crossreactivity: goat.

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