CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2)

CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation. \n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin/paraffin only): 1:30-1:60 \nStaining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min., followed by cooling at RT for 20 min.\nOptimal dilutions to be determined by the researcher\n\nPositive Control:\nTonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2253-05

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.09% sodium azide.

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG1,k

References

1. Davis S J and van der Merwe PA. The structure and ligand interactions of CD2:implications for T-cell function. Immunology Today. 17(4): 176-187 (1996). 2. Biondi A, Luciano A, Bassan R, et al. CD2 expression in acute promyelocytic leukemia is associated with microgranular morphology (FAB M3v) but not with any PML gene breakpoint. Leukemia. 9: 1461-1466 (1995). 3. Giegerich G W, Hein W R, Miyasaka M, et al. Restricted expression of CD2 among subsets of sheep thymocytes and T lymphocytes. Immunology. 66: 354-361 (1989). 4. Makgoba M W, Sanders M E and Shaw S. The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition. Immunology Today. 10(12): 417-422 (1989).

Additional Info

Recognizes human CD2 at 45-58kD.