CD2 is a group of antibodies which recognizes the T cell receptor for sheep red blood cells. This receptor is present on 80% of normal human peripheral blood lymphocytes, 95% of thymocytes, 100% of T lymphocytes and on a part of Natural Killer cells. Several epitopes can be distinguished on the target molecule. The combination of the monoclonal antibodies is strongly mitogenic in the presence of TPA.
Applications:
suitable for use in Immunofluorescence, flow cyytometry, Immunohistochemistry and the Study of T cell functions. Other applications have not been tested.
Recommended Dilutions:
Immunofluorescence: 2 microgram/5.1000000 cells/test
Flow cytometry: 2 microgram/5.1000000 cells/test
Immunohistochemistry: 1:25 to 1:50 only suitable on cryostat sections or cell smears
Optimal dilutions to be determined by the researcher.
Hybridoma:
Hybridization of NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer and 0.1% (w/v) sodium azide for long term storage. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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