Human CD2 is a T-lineage glycoprotein with roles in cell-cell adhesion and the transduction of activation signals. Multiple ligands for CD2 include CD58 (LFA-3), CD59, and CD48. CD2 may play a role in regulating T cell anergy.
Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 5x10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 min on ice with 80ul anti-CD2 antibody at 1ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.12 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.