Home  >  Products  >  CD20 (B1, B-Lymphocyte Antigen CD20, B-Lymphocyte Surface Antigen B1, Bp35, CD20, CVID5, LEU-16, Leukocyte Surface Antigen Leu-16, Membrane-Spanning 4-Domains Subfamily A Member 1, MGC3969, MS4A2, S7)
CD20 (B1, B-Lymphocyte Antigen CD20, B-Lymphocyte Surface Antigen B1, Bp35, CD20, CVID5, LEU-16, Leukocyte Surface Antigen Leu-16, Membrane-Spanning 4-Domains Subfamily A Member 1, MGC3969, MS4A2, S7)

CD20 (B1, B-Lymphocyte Antigen CD20, B-Lymphocyte Surface Antigen B1, Bp35, CD20, CVID5, LEU-16, Leukocyte Surface Antigen Leu-16, Membrane-Spanning 4-Domains Subfamily A Member 1, MGC3969, MS4A2, S7)

Cat no: C2270-02K

Supplier: United States Biological
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Human CD20 has four membrane-spanning domains and forms a homo-oligomeric calcium ion channel complex critical for normal B-cell function. CD20 is a B cell restricted antigen. Recognizes the CD20 molecule of 33/37kD. Inhibits B-lymphocyte differentation and induced Ig secretion. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Raji cells were washed and incubated 45 min on ice with 80ul of anti-CD20 at 10ug/ml. Cells were washed twice and incubated with Goat anti-mouse/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.89 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2270-02K
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. E.A. Clark, et al, (1985) PNAS USA 82:1766-1770, 2. J.A. Ledbetter, & E.A. Clark, (1986) Human Immunol 15:30-43, 3. J.P. Deans, et al, (1993) J Immunol 151:4494-4504, 4. T.F. Tedder & P. Engel, (1994) Immunol Today 15:450-454, 5. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 511-515.
Additional info: Recognizes human CD20.

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