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CD200 (CD200 Antigen, MRC OX-2 Antigen, Mox2, OX2, OX-2 Membrane Glycoprotein) (Low Endotoxin)

Cat no: C2548-96L


Supplier: United States Biological
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CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons. Recent studies have suggested that the CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation. Appications: Suitable for use in ELISA, Immunohistochemistry and Flow cytometry. Other applications have not been tested. Recommended Dilutions: Flow cytometry: 1:10-1:50 Use 10ul of the suggested working dilution to label 106 cells in 100ul. Optimal dilutions to be determined by the researcher. Endotoxin: (same/less than)0.01EU/ug Hybridoma: Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line Positive Control (Ihc): Mouse spleen Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2548-96L
Reactivities: Mouse
Hosts: Rat
Applications: ELISA, Flow Cytometry, Immunohistochemistry
Size: 500ug
Form: Supplied as a liquid in PBS. No preservatives added.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography. Endotoxin: (same/less than)0.01EU/ug
Alternative names: CD200 Molecule
References: 1. Hoek, R.M. et al. (2000) Down-regulation of the macrophage lineage through interaction with OX2 (CD200). Science 290: 1768-1771. 2. Nathan, C. and Muller, W.A. (2001) Putting the brakes on innate immunity: a regulatory role for CD200. Nature Immunology 3: 17-19.3. Broderick, C. et al. (2002) Constitutive retinal CD200 expression regulates resident microglia and activation state of inflammatory cells during Experimental Autoimmune Uvoretinitis. Am. J. Pathol. 161: 1699 - 1677.
Additional info: Recogizes mouse CD200 cell surface antigen.

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