CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons. Recent studies have suggested that the CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.
Appications:
Suitable for use in ELISA, Immunohistochemistry and Flow cytometry. Other applications have not been tested.
Recommended Dilutions:
Flow cytometry: 1:10-1:50 Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Endotoxin: (same/less than)0.01EU/ug
Hybridoma:
Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line
Positive Control (Ihc):
Mouse spleen
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.