CD200R1, Fc Chimera, Recombinant, Mouse (OX2R, OX2 Receptor)

CD200 R1, also known as OX-2 receptor, is a 90kD, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity.1-3 The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain.4 Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, 5-7 while its ligand, CD200, is widely distributed.8 Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders.9 Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains.10 The CD200 R-like molecules may interact differently with CD200.11, 12 The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals.13-15 CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12.4, 16\n\nA DNA sequence encoding the extracellular domain of mouse CD200 R1 (Met 1-Pro 236; Accession # BAC40777) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0\n\nCalculated molecular mass of 49.7kD. As a result of glycosylation, the recombinant monomer migrates as an ~ 90-100kD protein in SDS-PAGE under reducing conditions.\n\nActivity: Measured by its ability to bind rmCD200/Fc.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2548-96W11

Size

50ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute with PBS to (same/more than)100ug/ml.

Purity

(same/more than) 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.\nEndotoxin: (same/less than) 1EU/1ug.

References

1. Rosenblum, M.D. et al., 2006, J. Dermatol. Sci. 41:165. 9. Hoek, R.M. et al., 2000, Science 290:1768.\n2. Gorczynski, R.M., 2005, Curr. Opin. Invest. Drugs 6:483. 10. Hatherley, D. and Barclay, A.N., 2004, Eur. J. Immunol. 34:1688.\n3. Barclay, A.N. et al., 2002, Trends Immunol. 23:285. 11. Hatherley, D. et al., 2005, J. Immunol. 175:2469.\n4. Wright, G.J. et al., 2003, J. Immunol. 171:3034. 12. Gorczynski, R. et al., 2004, J. Immunol. 172:7744.\n5. Shiratori, I. et al., 2005, J. Immunol. 175:4441. 13. Zhang, S. and Phillips, J.H., 2006, J. Leukoc. Biol. 79:363.\n6. Cherwinski, H.M. et al., 2005, J. Immunol. 174:1348. 14. Zhang, S. et al., 2004, J. Immunol. 173:6786.\n7. Fallarino, F. et al., 2004, J. Immunol. 173:3748. 15. Jenmalm, M.C. et al., 2006, J. Immunol. 176:191.\n8. Wright, G.J. et al., 2001, Immunology 102:173. 16. Voehringer, D. et al., 2004, J. Biol. Chem. 279:54117.