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CD203a (SWC9)

Cat no: C2548-96X1


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): Neat Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2548-96X1
Reactivities: Porcine
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 2ml
Form: Supplied as liquid, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Supernatant
References: 1. McCullough, K. C. et al (1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages Vet Immunol Immunopathol 58 (3-4): 265 - 75, 2. McCullough K. C. et al (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection. Immunity. 98: 203 - 212, 3. Boersma, W. et al. (2001) Summary of workshop findings for porcine B cell markers. Vet. Immunol. Immunopathol. 80: 63 - 78, 4. Dominguez, J. et al. (1998) Workshop studies with monoclonal antibodies identifying a novel porcine differentation antigen SWC9. Vet. Immunol. Immunopathol. 60: 343 - 349, 5. Petersen, C.B. et al. (2007) Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. Dev. Comp. Immunol. 31:618-631, 6. Dominguez, J. et al. (1998) Porcine myelomonocytic markers: summary of the second international swine CD workshop. Vet. Immunol. Immunopathol. 60: 329 - 341.
Additional info: Recognizes porcine CD203a (SWC9) a cell surface antigen expressed on mature pig macrophages. Freshly isolated blood monocytes are SWC9 negative but do express SWC9 after culturing..

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