Home  >  Products  >  CD22 (B-Cell Receptor CD22, BL-CAM, B-Lymphocyte Cell Adhesion Molecule, FLJ22814, MGC130020, Sialic Acid-Binding Ig-like Lectin 2, SIGLEC2, Siglec-2, SIGLEC-2, T-Cell Surface Antigen Leu-14)
CD22 (B-Cell Receptor CD22, BL-CAM, B-Lymphocyte Cell Adhesion Molecule, FLJ22814, MGC130020, Sialic Acid-Binding Ig-like Lectin 2, SIGLEC2, Siglec-2, SIGLEC-2, T-Cell Surface Antigen Leu-14)

CD22 (B-Cell Receptor CD22, BL-CAM, B-Lymphocyte Cell Adhesion Molecule, FLJ22814, MGC130020, Sialic Acid-Binding Ig-like Lectin 2, SIGLEC2, Siglec-2, SIGLEC-2, T-Cell Surface Antigen Leu-14)

Cat no: C2272-92D

Supplier: United States Biological
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Human CD22 expression is restricted to normal Ig+ mature B cells and neoplastic B cells. CD22 is a transmembrane glycoprotein of 140kD with a predicted adhesion molecule function. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed paraffin Flow Cytometry: 5x10e5 cultured Raji cells were incubated 45 min on ice with 80ul of anti-CD22 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.17 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2272-92D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. D. Campana, et al, (1985) J Immunol 134:1524-1530, 2. J.L. Li, et al, (1989) Cell Immunol 118:85-99, 3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 190-193, 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 523-530, 5. D. Shan & O.W. Press, (1995) J Immunol 154:4466-4475.
Additional info: Recognizes human CD-22.

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