CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. It is lost upon terminal differentiation to plasma cells.
Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, and Western Blot. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.