CD22 is differentially expressed during B cell maturation. Initially, CD22 is expressed in the cytoplasm of pro-B and pre-B cells and then on the cell surface of mature B cells. CD22 expression is lost upon terminal differentiation to plasma cells. CD22 functions as an adhesion receptor, for leukocytes and erythrocytes, and a signalling molecule which may play a role in B-cell activation.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.