CD226, Fc Chimera, Recombinant, Mouse (DNAM1, DNAX Accessory Molecule-1)

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65kD type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2-4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (3-7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8-11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13-15).\n\nSource: A DNA sequence encoding the extracellular domain of mouse DNAM-1 (Met 1-Pro 254; Accession # Q8K4F0) was fused to the Fc region of mouse IgG2A via a polypeptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NS0.\n\nDefinition: Measured by its ability to bind biotinylated rmNectin-2.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 53.9kD protein migrates as an ~ 70-90kD protein in SDS-PAGE under reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M4699-02

Size

50ug

Applications

WB

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to (same/more than) 0.1mg/ml.

Purity

(same/more than) 80%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin (same/less than) 1EU.

References

1. Fuchs, A. and M. Colonna, 2006, Semin. Cancer Biol. 16:359.\n2. Tahara-Hanaoka, S. et al., 2005, Biochem. Biophys. Res. Commun. 329:996.\n3. Bottino, C. et al., 2003, J. Exp. Med. 198:557.\n4. Tahara-Hanaoka, S. et al., 2004, Int. Immunol. 16:533.\n5. Shibuya, A. et al., 1996, Immunity 4:573.\n6. Dardalhon, V. et al., 2005, J. Immunol. 175:1558.\n7. Bachelet, I. et al., 2006, J. Biol. Chem. 281:27190.\n8. Reymond, N. et al., 2004, J. Exp. Med. 199:1331.\n9. Kakehi, S. et al., 2007, Mol. Cell. Biochem. 301:209.\n10. Kojima, H. et al., 2003, J. Biol. Chem. 278:36748.\n11. Tahara-Hanaoka, S. et al., 2006, Blood 107:1491.\n12. Morimoto, K. et al., 2007, Oncogene July 16 [Epub ahead of print].\n13. Shibuya, K. et al., 1999, Immunity 11:615.\n14. Shibuya, K. et al., 2003, J. Exp. Med. 198:1829.\n15. Shibuya, A. et al., 1998, J. Immunol. 166:1671.