CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.
Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
NS-1 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab