CD23

Cat no: C2273-93K

Supplier: United States Biological

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Description
Additional Information

Human CD23 is a low affinity receptor for IgE (Fc epsilon RII) found primarily on mature B cells and also on eosinophils, monocytes, dendritic cells, platelets, and a subset of thymic epithelial cells. CD23 interacts with CD21. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed paraffin Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and preincubated 5 min with 20ul of 250ug/ml human IgG (To block non specific binding) after which they were1.2 washed and incubated 45 min on ice with 80ul of anti-0.2 0. Binding of anti-CD23 antibody to human cell lines 0 10 20 30ug/ml PBL Nalm-6 1 0.8 0.6 CD23 antibody at a concentration of 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 5% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C2273-93K
Reactivities:	Human
Hosts:	Mouse
Applications:	ELISA
Size:	100ug
Form:	Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride.
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein A affinity chromatography.
References:	1. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 67-81, 2. D. L. Hardie, et al, (1993) Eur J Immunol 23:997-1004, 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 530-538, 4. J.Y. Bonnefoy, et al, (1995) Curr Opin Immunol 7:355-359.
Additional info:	Recognizes human CD23.

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CD23

Cat no: C2273-93K

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