CD23 (IgE Receptor gp45)

CD23 is a low affinity receptor for IgE (FceR) and both the FceRIIa and FceRIIb are expressed on mature IgD+/IgM+ B cells. The FceRIIb form is also present on monocytes, activated macrophages, eosinophils, platelets and follicular dendritic cells. CD23 is also present in a soluble form in serum. CD23 together with CD19 and CD22, plays a role in the modulation of B cell activation through membrane immunoglobulin and is involved in regulation of IgE synthesis. Expression of CD23 is increased by IL-4 and down-regulated by B-cell growth factor (BCGF). Binding of IgE immune complexes to CD23 increases the efficiency of B cell antigen processing and presentation. Via its interaction with CD21 it may play a role in adhesion processes. It mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. \n\nCD23 is a 45kD glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B\ncells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic\nlymphocyctic leukemia and some cases of centroblastic/centrocytic lymphoma.\n\nThe CD23 clustered antibodies give a characteristic binding pattern with follicular dendritic cells but the antigen is absent on plasma cells and B cell progenitors. Expression of the CD23 antigen is increased as B lymphocytes pass from a resting to an active phase. Cleavage of the membrane bound form releases a series of soluble fragments all of which still bind IgG except for the 12kD moiety.\n\nApplications: Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.\n\nRecommended Dilution: 1:20-1:40 \nOptimal dilutions to be determined by the researcher.

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SPECIFICATIONS

Catalog Number

C2274-03

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as concentrated tissue culture supernatant in 15mM sodium azide.

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG1,k

References

1. Kaiserlian D,Lachaux A,Grosjean I,et al.intetestinal epithelial cells express the CD23/FceRII molecule:enhanced expression in enteropathies. Immunology.80:90-95(1993).\n2. Aubry J P,Bonnefoy J Y, DeVries J E, et al.The CD23 antigen is the human lymphocyte receptor for IgE. In:McMichael A J,et al. Eds.Leucocyte Typing IIII.While cell differentiation Antigens.Oxford University Press-Oxford,New York,Tokyo.417-419(1987).\n3. Kikutani H,Suemura M,Owaki H,et al. CD23 is a low

Additional Info

Recognizes CD23 at 45kDa. Species Reactivity: Human. Does not react with Rat.