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CD24 (Low Endotoxin)

CD24 (Low Endotoxin)

Cat no: C2275-06A

Supplier: United States Biological
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Applications: Suitable for use in Flow cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or cells or 100ul whole blood. Immunohistochemistry: frozen tissues. Not suitable for use on paraffin tissues. Optimal dilutions to be determined by the researcher. Endotoxin Level: <0.1EU/ug Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of a mouse myeloma cell line. Positive Control Tissue: Tonsil Recommended Secondary Antibodies: I1904-65W: IgG, F(ab�)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab�)2X-adsorbed (HRP) Pab Rb xMo I1904-66A: IgG, F(ab�)2 (FITC) Pab Rb xMo Recommended Negative Controls IgG1 Murine Negative Control: Low Endotoxin Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2275-06A
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 500ug
Form: Supplied as a liqquid in PBS, pH 7.4. No preservatives or stabilizers added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Barcos, M. et al. (1986) Haematol. Oncol. 4:251-259. 2. Fukukawa, T. et al. (1986) Exp. Hematol. 14:850-855.
Additional info: Recognizes human CD24 cell surface antigen, a heavily glycosylated molecule of 35-45kD, expressed by granulocytes and B lymphocytes, and by some activated T cells and T cell malignancies.

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