Applications:
Suitable for use in Flow Cytometry, ELISA, and Western Blot. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 3-10ug/ml per 10e6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.
ELISA: 0.1-0.4ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.