Home  >  Products  >  CD25 (CD25, IDDM10, IL2R, IL2-RA, IL-2-RA, IL-2 receptor subunit alpha, IL-2R subunit alpha, Interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR)
CD25 (CD25, IDDM10, IL2R, IL2-RA, IL-2-RA, IL-2 receptor subunit alpha, IL-2R subunit alpha, Interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR)

CD25 (CD25, IDDM10, IL2R, IL2-RA, IL-2-RA, IL-2 receptor subunit alpha, IL-2R subunit alpha, Interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR)

Cat no: C2275-82J

Supplier: United States Biological
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Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells (4). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM). 5x10e5 cells per tube were washed and incubated 45 min on ice with 80ul of anti-CD25 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2275-82J
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. L.A. Rubin, et al, (1985) Hybidoma 4:91-102. 2. B. Boutin, et al, (1989) Neuropediatrics 20:202-206, 3. T.A. Waldmann, et al, (1989) Annu Rev Biochem 58:875-911, 4. T.A. Waldmann, et al, (1995) Blood 86:4063-4075, 5. U.S. Patent No. 4,707,443, 6. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 403-406.
Additional info: Recognizes epitope B of the human IL- 2 alpha receptor of about 55kD (1,6). Does not block IL-2 binding to its receptor (1).

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