CD27 (CD27 Antigen, CD27L Receptor, MGC20393, S152, T14, T-Cell Activation Antigen CD27, TNFRSF7, Tp55, Tumor Necrosis Factor Receptor Superfamily Member 7)

Cat no: C2379-10N

Supplier: United States Biological

0 reviews | Write a Review Pencil

Read More on Supplier Website

Description
Additional Information

Human CD27 is a lymphocyte specific member of the tumor necrosis factor (TNF) receptor family and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD27 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 64% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C2379-10N
Reactivities:	Human
Hosts:	Mouse
Applications:	ELISA
Size:	100ug
Form:	Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein A affinity chromatography.
References:	1. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 350-352, 2. K. Agematsu, et al, (1994) J Immunol 153:1421-1429, 3. R.Q. Hintzen, et al, (1994) Immunol Today 15:307-311, 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 356-360, 435-437, 5. K. Agematsu, et al, (1995) J Immunol 154:3627-3635.
Additional info:	Recognizes human CD27.

See Full Product Listings >

CD27 (CD27 Antigen, CD27L Receptor, MGC20393, S152, T14, T-Cell Activation Antigen CD27, TNFRSF7, Tp55, Tumor Necrosis Factor Receptor Superfamily Member 7)

Cat no: C2379-10N

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from United States Biological




By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*