CD273 (B7-DC, Programmed Death Ligand 2, PD-L2)

Cat no: C2549-21A

Supplier: United States Biological

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Description
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CD273 is a single-pass type 1 membrane protein belonging to the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1). CD273 is highly expressed in the tissues of the heart, liver, lung, placenta and pancreas. It is expressed in dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells. CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is particularly important in the maintenance of self tolerance. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml Immunohistochemistry (Paraffin): 2.5ug/ml. Antigen retrieval by heating in sodium citrate buffer, pH 6.0 is required. Optimal dilutions to be determined by the researcher Positive Control Tissue (IHC): Mouse brain Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C2549-21A
Reactivities:	Human, Mouse, Rat
Hosts:	Rabbit
Applications:	Immunohistochemistry, Western Blot
Size:	100ug
Form:	Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by affinity chromatography.
References:	1. Zhang Y, et al. (2006) Regulation of T cell activation and tolerance by PDL2. PNAS 103:11695-700 .2. Adriana J, et al (2006) Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 67: 568-578. 3. Ishida Y, et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11: 3887-3895. 4. Holling TM, et al. (2004) Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 65: 282-290.
Additional info:	Recognizes human CD273; band observed at ~ 30kD in Raji cell lysate. Species Crossreactivity: mouse and rat

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CD273 (B7-DC, Programmed Death Ligand 2, PD-L2)

Cat no: C2549-21A

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