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CD28

Cat no: C2380-16


Supplier: United States Biological
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The ligand for CD28 is CD80 (B7) which is found on activated B cells suggesting a role for CD28 in T and B cell interaction. Applications: Suitable for use in Flow Cytometry, T cell proliferation and in cell stimulation studies where it has been suggested that measuring T cell function in HIV infected asymptomatic patients may be an early indicator of AIDS, independent of CD4+ cell counts. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2380-16
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 20mM Tris, pH 8.0, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by ion exchange chromatography following ammonium sulfate precipitation.
References: 1. Leucocyte Typing IV, Oxford University Press (1989) 2. Leucocyte Typing III, Oxford University Press (1987) 3. Lier, R. A. van, et al. Eur. J. Immunol. 18, 167 1988)
Additional info: Recognizes the 44kD antigen found on a sub-population of T-cells and activated B cells. These CD28+ CD8 cells are now known to be cytotoxic T-cell precursors. Will induce the proliferationof resting T lymphocytes. Does not react with B cells, granulocytes or monocytes. Does not crossreact with canine.

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