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CD28 (MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein CD28, Tp44)

CD28 (MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein CD28, Tp44)

Cat no: C2380-01G

Supplier: United States Biological
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CD28 is a 44kD glycoprotein also known as Tp44 or T44. It is a member of the immunoglobulin superfamily and is expressed on thymocytes, a/bTCR+ T cells, most g/dTCR+ T cells, and an NK cell subset. During interaction with CD80 (B7-1) or CD86 (B7-2), CD28 acts as the second signal for T cell stimulation. Applications: Suitable for use in Flow Cytometry, Functional Assays, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2380-01G
Reactivities: Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation
Size: 500ug
Form: Supplied as a liquid in PBS, pH 7.4. No preservatives added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Tacke, M. et al. (1995) Cellular distribution and costimulatory function of rat CD28. J. Immunol. 154: 5121-5127, 2. Tacke, M. et al. (1997) CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur. J. Immunol. 27:239-247.
Additional info: Recognizes rat CD28.

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