CD28 is a 44kD glycoprotein also known as Tp44 or T44. It is a member of the immunoglobulin superfamily and is expressed on thymocytes, a/bTCR+ T cells, most g/dTCR+ T cells, and an NK cell subset. During interaction with CD80 (B7-1) or CD86 (B7-2), CD28 acts as the second signal for T cell stimulation.
Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: (same/less than)0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.