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CD28 (MGC138290, T-Cell-Specific Surface Glycoprotein CD28, Tp44, TP44)

CD28 (MGC138290, T-Cell-Specific Surface Glycoprotein CD28, Tp44, TP44)

Cat no: C2380-01L

Supplier: United States Biological
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Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28 identified as CD80(B7-1) and CD86(B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60kD and 80kD respectively. CD28 antibodies have recently been shown to have a strong adjuvant effect when conjugated to the immunogen (4). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured HPB-MLT cells per tube were washed and incubated 45 min on ice with 80ul of anti-CD28 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.26 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2380-01L
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. M.K. Jenkins & J.G. Johnson, (1993) Curr Opin Immunol 5:361-367. 2. 61st Forum in Immunology, (1995) Res Immunol 146:127-205, 3. I. Kariv, A. Truneh & R.W. Sweet, (1996) J Immunol 157:29-38, 4. J. Carling, et al, (2003) J Immunol 33:135-142.
Additional info: Recognizes CD28 expressed on human T cells and also on BHK cells transfected with human CD28. Monomeric and cross linked CD28 will stimulate expression of IL-2 from CD28+ cells.

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