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CD29 (Fibronectin receptor subunit beta, FNRB, GPIIA, Integrin beta-1, MDF2, MSK12, VLA-4 subunit beta, VLAB, VLA-BETA)

CD29 (Fibronectin receptor subunit beta, FNRB, GPIIA, Integrin beta-1, MDF2, MSK12, VLA-4 subunit beta, VLAB, VLA-BETA)

Cat no: C2381-03L

Supplier: United States Biological
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Human CD29 is the beta subunit of an integrin family of molecules expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. CD29 can form heterodimer pairs with at least nine different alpha subunits. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed paraffin Flow Cytometry: 5x10e5 cultured human HPB-MLT cells were washed and incubated 45 min on ice with 80ul of anti-CD29 antibody at a concentration of 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2381-03L
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1612-1613.
Additional info: Recognizes human CD29 integrin subunit.

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