CD29, also known as Integrin beta 1, is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. Interaction between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:500-1:1,000
Western Blot: 1:500-1:1,000
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD29 protein.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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