CD29 (Integrin beta 1) (FITC)

Integrin beta 1, also known as CD29, is a 130kD transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow cytometry: 2ug/ml. 20ul reagent/100ul whole blood\nImmunoprecipitation: 1:50\nWestern Blot: 2ug/ml non-reducing conditions recommended.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C2381-10W

Size

100 tests

Applications

FC, IP, WB

Hosts

Mouse

Reactivities

Hum, Can, Prc

Form

Supplied as a liquid in PBS, pH 7.4, BSA, 15mM sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Leukocyte Typing VI. Kishimoto, T., et al. (Eds.), Garland Publishing Inc. (1997). 2. Simova S, Klima M, Cermak L, Sourkova V, Andera L. : Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis;13(3):423-36. 2008 Mar. [PMID: 18165900]

Additional Info

Recognizes human Integrin beta 1. Species Crossreactivity: canine and porcine. Does not react with mouse.

Alternative Names

beta 1 subunit, CD29 antigen, Fibrinogen Receptor beta subunit, GPIIA, ITGB1, MDF2, Very Late Activation Protein beta Polypeptide, VLAB, VLAbeta