CD29, Recombinant, Human (Integrin alpha 10 beta 1, Integrin, beta1, CD29, Very Late Antigen, VLAb1, Platelet gplla, ITGB1, Fibrinogen Receptor beta Subunit, FNRB, Integrin VLA4 Subunit beta, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, VLA

Integrin a10b1 is one of twelve integrin family adhesion receptors that share the b1 subunit. The non-covalent heterodimer of 160kD a11 and 130kD b1/CD29 type I transmembrane glycoprotein subunits is expressed mainly on chondrocytes within cartilage, but also infibrous connective tissues such as heart valves and ligaments. The a10 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site. The b1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail . The dimer is folded when it is least active. Divalent cations and intracellular (inside-out ) signaling convert it to its most active, extended and open conformation. The 1100aa human a10 extracellular domain (ECD) shares 88-92% aa sequence identity with mouse, rat , canine and bovine a10, while the 708aa human b1 ECD shares 92-96% aa sequence identity with rat , bovine, mouse, and feline b1. A reported a10 splice variant lacking ECD aa974-1012 was not expressed on the cell surface. I domain-containing b1 integrins a1b1, a2b2, a10b1 and a11b1 all bind collagens; all but a11b1 also bind laminins. During cartilage differentiation, a10b1 is thought to be the main integrin binding type II and IX cartilage collagens. However, deletion of mouse a10 causes a mild phenotype including slightly shortened bones and narrowed hypertrophic zones, indicating that another collagen-binding integrin, likely a2b1, may compensate for a10b1 functions. Migration of melanoma cells has been noted to correlate with a10b1 expression.\n\nSource:\nChinese Hamster Ovary cell line, CHO-derived. Gln17-Gly523, with a C-terminal 6-His tag.\n\nMolecular Weight: 160-175kD & 125-150kD (SDA-PAGE, reducing conditions) \n\nBiological Activity:\nMeasured by its binding ability in a functional ELISA. When mouse Laminin is coated at 10ug/ml, Integrin a10b1 binds with an apparant Kd<5nM. \n\nEndotoxin: (same/less than)1EU/ug (LAL) \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with 200ug/ml PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2381-02N

Size

50ug

Form

Supplied as a lyophilized powder from PBS. Carrier free. Reconstitute with 200ug/ml PBS.

Purity

(same/more than)95% (SDS-PAGE)

References

1. Takada, Y. et al. (2007) Genome Biol. 8:215. 2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619. 3. Camper, L. et al. (1998) J. Biol. Chem. 273:20383. 4. Camper, L. et al. (2001) Cell Tiss. Res. 306:107. 5. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206. 6. Bengtsson, T. et al. (2001) Matrix Biol. 20:565. 7. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139. 8. Popova, S.N. et al. (2007) Acta Physiol. 190:179. 9. Varas, L. et al. (2007) Stem Cells Dev. 16:965. 10. Gigout, A. et al. (2008) J. Biol. Chem. 283:31522. 11. Bengtsson, T. et al. (2004) J. Cell Sci . 118:939. 12. Wenke, A.K. et al. (2007) Cell Oncol. 29:373.