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CD3

CD3

Cat no: C2253-38D

Supplier: United States Biological
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The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intracytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells and is present in majority of T cell neoplasms. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells or 100ul whole blood. Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment. Citrate biffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2253-38D
Reactivities: Canine
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. McDonough, S. P. etal (2000) Vet Pathol. 2000 Nov;37:637-46. 2. Moore, P. F., Affolter, V. K. and Vernau, W. (2006) Vet Pathol. 2006 Sep;43:632-45. 3. Vernau, W and Moore, P. F. (1999) Vet Immunol Immunopathol. 69:145-64. 4. Moreno, J. et al (1999) Vet Immunol Immunopathol. 71:181-95. 5. Byrne, K. et al (2000) Vet Immunol Immunopathol. 73:167-82. 6. Machado, G.F. et al. (2011) Braz J Vet Pathol 4: 47-51 7. Fellman, C.L. et al. (2011) Vet Immunol Immunopathol. 140: 175-80. 8. Watabe, A. et al. (2011) Vet Immunol Immunopathol. 142: 189-200.9. Hsiao, Y.W. et al (2004) J Immunol. 172: 1508-14. 10. Huang, Y.C. et al. (2008) J Leukoc Biol. 84: 1501-10. 11. Out, T.A. et al. (2002) Immunology. 105: 499-508. 12. Zentek, J. et al. (2002) J Nutr. 132: 1652S-4S. 13. Hai, M. et al. (2008) Gene Ther. 15: 1067-71. 14. Altmann, S. et al. (2008) Vet Immunol Immunopathol. 126: 367-72. 15. Ting-De Ravin, S.S. et al. (2006) Blood. 107: 3091-7. 16. Miranda, S. et al. (2007) Vet Parasitol. 144: 251-60.
Additional info: Recognizes the canine CD3 cell surface antigen, expressed by mature T lymphocytes.

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