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CD3

CD3

Cat no: C2253-38H

Supplier: United States Biological
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The CD3 antigen appears in the cytoplasm of the cell during the early stage of T cell development and is expressed on the cell membrane at the late stage. CD3 is present on 60-80% of normal peripheral blood lymphocytes and 60-70% of thymocytes and plays an important role in the signal transduction after antigen recognition by TCR. This monoclonal antibody has a strong mitogenic effect on T lymphocyte proliferation (in soluble or immobilized conditions) and has an immunosuppressive effect at a high dose. In addition, NK cells express CD3 in the cytoplasm and this antibody provides a tool for analyzing the relationship and the common precursor of both T and NK cells. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:5 Optimal dilutions to be determined by the researcher. Hybridoma: P3/NS1/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab
Catalogue number: C2253-38H
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 2ml
Form: Supplied as a liquid in 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Supernatant
References: 1. Beverley, P.C.L. and Callard, R.E. (1981) Eur. J. Immunol. 11: 329-334. 2. Zarkesh-Esfahani, H. et al. (2001) J. Immunol. 167: 4593 - 4599. 3. Kung, P. et al. (1979) Science. 206: 347-9. 4. Clevers, H. et al. (1988) Eur. J. Immunol. 18: 705-10. 5. Clark, E.A. et al. (1983) Immunogenetics 18: 599-615. 6. Clevers, H. et al. (1988) Annu. Rev. Immunol. 6: 629-62. 7. Meuer, S.C. et al. (1983) Nature 303: 808-10. 8. Clark, E.A. et al. (1989) Immunology Today 10: 225-8. 9. Campana, D. et al. (1987) J. Immunol. 138: 648-55. 10. Erber, W.N. et al. (1984) Lancet 1(8385): 1042-6. 11. Erber, W.N. et al. (1986) Lancet 1(8485): 761-5. 12. Denning, S.M. et al. (1987) Activation of human thymocytes via CD3 and CD2 molecules. In McMichael, A.J. et al. Leucocyte Typing III: White Cell Differentiation Antigens. Edited by Oxford University Press pp 144-7. 13. Grogan, T.M. et al. (1985) P Am. J. Clin. Pathol. 83: 271-88. 14. Maggiorella, M. et al. (1998) Blood. 91 (9): 3103 - 3111.
Additional info: Recognizes human CD3, a surface protein of peripheral blood T cells at ~19kD. Present on more than 95% of circulating human peripheral T cells. Species Crossreactivity: chimpanzee

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