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CD3 (Early T Cell Marker, T Cell receptor Complex, TCR) (Biotin)

CD3 (Early T Cell Marker, T Cell receptor Complex, TCR) (Biotin)

Cat no: C2254-08H

Supplier: United States Biological
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CD3e is a 20kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3e, one CD3g, one CD3d, one CD3z (CD247), and a T-cell receptor (a/b or g/d) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The HIT3a antibody is able to stimulate T cell activation. Applications: Suitable for use in Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: (same/less than)0.5ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2254-08H
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Conjugates: Biotin
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by immunoaffinity chromatography.
References: 1.Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp, W.1989. Leucocyte Typing IV. Oxford University Press, New York. 3. Barclay, N., et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. 4. Sedelies, K. A., et al., 2004. J. Biol. Chem. 279:26581. 5. Rivollier, A., et al., 2004. Blood 104:4029. 6. Scharschmidt, E., et al., 2004. Mol. Cell Biol. 24:3860. 7. Smeltz, R. B., 2007. J. Immunol. 178:4786.
Additional info: Recognizes human CD3.

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