CD3 (Early T Cell Marker, T Cell Receptor Complex, TCR, T-cell Surface Glycoprotein CD3)

The CD3 antigen is present on early thymocytes and native T cells and is generally regarded as a Pan T cell marker. This antibody will help detect CD3 expression in normal and uncharacterized tissues.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen): 1:100-1:200 for 1 hour at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTonsil, membrane staining pattern.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2254-01J

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1Clark E A and Ledbetter J A. Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase. Immunology Today. 10:225-28 (1989). 2.Clevers H, Alarcon B, Wileman T, et al.. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Review of Immunology. 6:629-662 (1988). 3. Clevers H, Dunlap S and Terhorst C. The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. European Journal of Immunology. 18:705-710 (1988). 4.Campana D, Thompson J S, Amlot P, et al.. The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. Journal of Immunology. 138:648-655 (1987). 5.Denning S M, Tuck D T, Singer K H, et al.. Activation of human thymocytes via CD3 and CD2 molecules. In: McMichael AJ, et al.. eds. Leucocyte Typing III, White Cell Differentiation Antigens. Oxford-New York-Tokyo. Oxford University Press. 144-147 (1987). 6.Meuer S C, Acuto O, Hussey R E, et al.. Evidence for the T3-associated 90k heterodimer as the T cell antigen receptor. Nature. 303:808-810 (1983). 7.Beverley P C L and Callard R E. Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. European Journal of Immunology. 11:329-334 (1981).

Additional Info

Recognizes human CD3.

Alternative Names

CD247, T-cell Surface Glycoprotein CD3 delta Chain, CD3d, T-cell Receptor T3 delta Chain, T3D, T-cell Surface Glycoprotein CD3 gamma Chain, CD3g, T-cell Receptor T3 gamma Chain, T3G, T-cell Surface Glycoprotein CD3 epsilon Chain, CD3e, T-cell Surface Antigen T3/Leu-4 epsilon Chain, T3E, T-cell Surface Glycoprotein CD3 zeta Chain, CD3z, T-cell Receptor T3 zeta Chain, T3Z, TCRZ